A Laboratory Course in Tissue Engineering by Melissa Kurtis Micou

By Melissa Kurtis Micou

Filling the necessity for a lab textbook during this swiftly growing to be box, A Laboratory path in Tissue Engineering helps scholars strengthen hands-on adventure. The publication comprises fifteen standalone experiments according to either vintage tissue-engineering techniques and up to date advances within the box. Experiments surround a suite of generally appropriate innovations: mobile tradition, microscopy, histology, immunohistochemistry, mechanical checking out, smooth lithography, and customary biochemical assays. as well as educating those particular innovations, the experiments emphasize engineering research, mathematical modeling, and statistical experimental layout.

A reliable beginning in Tissue Engineering—and communique Skills

Each scan comprises history info, studying pursuits, an summary, defense notes, an inventory of fabrics, recipes, equipment, pre- and postlab questions, and references. Emphasizing the significance for engineering scholars to advance powerful conversation abilities, every one test additionally incorporates a facts research and reporting part that offers a framework for succinctly documenting key effects. A separate bankruptcy presents guidance for reporting ends up in the shape of a technical record, magazine article, prolonged summary, summary, or technical poster.

Customize Your classes with greater than a Semester’s worthy of Experiments

The e-book is a handy resource of educational fabric acceptable for undergraduate or graduate scholars with basic wisdom of engineering and mobilephone biology. all the experiments were commonly established to enhance the possibility of profitable info assortment. additionally, to reduce lab charges, the experiments make broad use of apparatus quite often present in laboratories built for tissue tradition. A ideas handbook, on hand with qualifying path adoption, comprises solutions to pre- and postlab questions, prompt gear providers and product numbers, and different assets to assist plan a brand new tissue engineering course.

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Extra resources for A Laboratory Course in Tissue Engineering

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Dispensing. Rest the tip of the serological pipette against the inner wall of the receptacle (if possible) and deliver the liquid smoothly by pressing the lower button on the handle. • Pipetting organic solvent. Glass serological pipettes must be used to pipette organic solvents as many of these reagents will dissolve pipettes made of polystyrene. You can distinguish a glass pipette by the weight compared to polystyrene pipettes (glass is heavier and will “clink” when bounced on a hard surface).

15. Discard the cryogenic vial and the 15 mL tube into the biohazard waste. Discard the Pasteur pipette into the biohazard sharps waste. Store the complete DMEM at 4°C. Wipe down the BSC with 70% ethanol. 6 Refeeding Cell Cultures/Changing Media As cultured cells proliferate, nutrients in the media are gradually depleted and waste accumulates. To maintain the health of the culture, the media needs to be changed every 2 or 3 days. Some culture media contain phenol red, an indicator of pH. Media that have changed color from red to yellow have overgrown and been depleted of essential nutrients or become infected and should be discarded.

Alternatively, when working in the biosafety cabinet, liquid biohazardous waste can be aspirated with a Pasteur pipette and collected in a glass vacuum flask. Before the flask reaches two-thirds full, the contents should be disposed of properly. Depending on institutional guidelines, bleach can be added to the flask before connecting it to the vacuum source so that waste is treated immediately when aspirated into the flask. If your institution does not allow bleach in the vacuum trap, the liquid waste should be treated with 10% bleach for 30 minutes and then poured down the drain with running water.

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