A Textbook of Practical Physiology by C. L. Ghai

By C. L. Ghai

With the writing of the eighth version of this famous and renowned booklet on useful body structure, it has accomplished over 25 years of improvement. the scholars have regularly demanded simplicity, directness, and readability of expression that's inside of their effortless take hold of. it's was hoping that the ebook meets with their standards. The ebook conforms to the syllabi and classes mandated by means of the clinical and Dental Councils of India. As in step with their new curriculum coverage, the sections on Hematology, Human Experiments, and scientific exam, were enormously accelerated and up-to-date and lots of new figures and diagrams extra. The part on Animal Experiments has been curtailed. The Appendix offers an enormous volume of Physiological info. a brand new characteristic has been the inclusion of OSPEs (Objective established sensible Examinations) for assessing scholars’ ability in functional body structure examinations. intended basically for MBBS, BDS, B. Ph-Th scholars, the ebook will end up worthy to scholars of BSc and MSc (Physiology), BSc and MSc (Pharmacy), MD (Physiology) and people learning Human body structure in lifestyles Sciences faculties and Universities.

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Since the living cells are mostly transparent, they must be stained with vital stains, or they must be first fixed in 11 alcohol and then stained with acid or basic dyes before they can be viewed under the microscope. In this microscope, a special phase plate is inserted into the condenser, which can retard the speed of some light waves. Since the tissue cells and organisms have different refractive indices, this microscope uses these differences to produce an image with good contrast of light and shade.

5 mm in OI lenses respectively. Figure 1-4 shows the approximate working distances for each lens. Note that the OI lens has to be immersed in a drop of oil. Low power objective (10 ×) = 10 × 10 = 100 times. High power objective (45 ×) = 45 × = 450 times. Oil immersion objective (100 × ) = 100 × 10 = 1000 times. 3. PROTOCOL (PROCEDURES) FOR THE USE OF MICROSCOPE Principle A focused beam of light passes through the material under study into the microscope. Parts of the specimen that are optically dense and having a high refractive index or are colored with a stain (dye), cast a potential shadow which is magnified in 2 main stages as it passes into the observer’s eye.

See page 22. 8 Why should the pricked finger not be squeezed? What is meant by free-flowing blood and why should it be preferred over squeezed blood? Squeezing or pressing the finger contaminates blood with tissue fluid, (thus diluting the blood and giving low values), and epithelial and/or endothelial cells. Free-flowing blood is that which comes out of the wound without pressing or squeezing. Hematology 1-4 23 Hemocytometry (Cell Counting) The Diluting Pipettes STUDENT OBJECTIVES After completing this experiment, you should be able to: 1.

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